Types of peptide libraries

Alanine Scanning Library

We sequentially substituted Ala for amino acids in the sequence; By scanning peptide libraries with Ala, the effects of specific amino acids on biological activities such as structure and function of the whole protein can be identified.

Positional Scanning Library

Single site scanning peptide library is an important tool for optimizing peptide sequences. The selected region or site of the polypeptide was systematically replaced with other amino acids, and the optimal amino acid at that site was determined by peptide activity assays. Such peptide libraries help researchers to discover specific regions with special effects or activities at specific locations.

Overlapping Library

Peptide libraries are commonly used to screen linear or continuous epitopes. Peptide library design mainly consists of peptide length design. Two parameters (peptide length and offset number) are determined. It is likely that an Orphan peptide will be left when the protein starts at the N terminus and gradually truncates to the C terminus. To maintain a consistent length, we suggest transferring the N terminus by one or a few amino acids.

Types of peptide libraries

Truncation peptide libraries

The truncated peptide library was used to confirm the shortest amino acid sequence in the peptide sequence. Truncated peptide libraries are usually created by systematically removing the amino acids flanking the original polypeptide sequence. If some important amino acids are known, these amino acid sequences should be retained and then the screen should be started by removing amino acids one by one from the other end of the peptide.

Random Peptide Library

The Random polypeptide library designed 20 natural amino acids by the shotgun method. shotgun approach. At the same time, the selected amino acid residues were randomly substituted.

Scrambled Library

The Scrambled Library design was based on internal substitution of the amino acid sequence of the original polypeptide. It is the most mutated peptide library, and disrupted peptides are often used as negative controls to demonstrate that a particular sequence is critical for protein function or activity. It is also a random screening tool for finding new targets.