The Gutuo biological experimenter taught you how to use the liquid chromatograph

Liquid chromatograph is a user-centered intelligent chromatograph, which has the basic performance of conventional HPLC, and extends more intelligent functions. It can well meet the different application requirements of users, so that users can use it more easily and obtain accurate analysis data.

First, the principle:

The principle of high performance liquid chromatography is based on the original classical chromatography on the basis of the meteorological color harmonic theory, the column is used in a special way with small particles, the result is that the column efficiency is much higher than the original classical liquid color harmonic, it can also have a highly sensitive detector after the use of the column. Enables continuous detection of the outgoing analyte.

The chromatograph is the use of the mixture of components in the fixed phase and mobile phase dissolution, distribution or adsorption of chemical interaction performance differences, so that the components in the relative motion of the two phases repeatedly subjected to the above forces to achieve mutual separation. High performance chromatographs are widely used in food analysis, environmental analysis, life science, medical laboratory and inorganic analysis. “In general, 80 to 85 percent of organic matter can in principle be analyzed by HPLC.”

Ii. Instrument Use:

Chromatograph is a commonly used chromatograph product. It is an instrument that separates the mixture first and then analyzes and identifies the mixture by using the difference in the distribution ratio between liquid-solid or insoluble two liquids. In the known organic compounds, about 80% can be separated and analyzed by high-performance liquid chromatography, and because the conditions of this method are mild, It does not destroy the sample, so it is especially suitable for organic compounds and living substances with high boiling point, difficult gatizing and volatilization, and poor thermal stability.

Liquid chromatograph is widely used in biochemistry, food analysis, pharmaceutical research, environmental analysis, inorganic analysis and other fields. It can control all parameters and operation of the chromatograph, implement editing function, and automatically analyze sequence samples. Real-time online display chromatogram, integration and report analysis results, drawing standard curve, etc.


Post time: May-26-2023