High-throughput polypeptide array technology principle

Peptide array is a new type of biochip, which is the most direct research technology to reveal various diseases and biochemical phenomena in the post-gene era. In this technology, peptides of any designed amino acid sequence are synthesized in situ at high density on specially processed chips. Each chip can host thousands or more peptides. This technique is mainly used to detect the binding sites/domains of proteins with other proteins or substances, such as polysaccharides, nucleic acids, compounds, etc.

Array technology can synthesize peptide amino acid sequences in situ according to the known amino acid sequence of the protein according to the principle of sequence drift, or can synthesize amino acid sequences arbitrarily according to the structure of the substance to be tested, and arrange these peptides in a certain order at a high density on the chip. Then, the material to be measured by immune detection technology and chip in response, find out the binding reaction with the material being tested loci/domain structure, and then input the data into the computer, the three-dimensional structure of the protein, binding sites of proteins and the material being tested.

High-throughput polypeptide array technology principle

Peptide array technology is widely used in basic scientific research, drug screening, target identification, epitope mapping, vaccine development, diagnostic kit development, structure and function research, and so on. At the same time, due to the increasing interest in polypeptide drugs increasingly, the technology can also meet a large number of pharmaceutical companies growing demand for more peptide drug development.

Peptide arrays as a kind of high flux research tools, is a new type of biological chip, which USES automation equipment, by in situ synthesis technology, will be hundreds or even thousands of specially designed peptides placed in the carrier of high density matrix of the special processing, such as glass or filter paper. Use of the biological chip, scientists can directly reveal the secret of the interaction between proteins and other biological macromolecules.