Peptide chemical synthesis of choice is basically solid phase peptide synthesis (SPPS), can accept ordinary synthesis of the vast majority of any kind of peptide sequence, mainly containing complex or cyclic peptide products. However, synthetic strategies considering solid-phase peptide synthesis mainly suffer from adverse environmental impacts due to the extensive use of solvents. Most of the solvents commonly used in peptide chemistry have long been considered problematic solvents by environmental agencies in various countries and need to be replaced immediately, which has sparked a movement in academia and industry in recent years to make peptide synthesis greener. Such efforts have focused on solvent substitution, recycling and reduction, and research on substitution synthesis methods.
One drawback of peptide therapy is its poor oral bioavailability, which severely limits oral delivery. This drawback can generally be addressed by alternative routes of administration, such as subcutaneous injection or inhalation, but advances in peptide formulation, such as the use of permeability enhancers to enhance oral absorption, will clearly accelerate the growth of this important therapeutic molecule.
In recent years, most of the reagents and solvents used in peptide chemistry have been defined as environmentally polluting ingredients by ECHA(European Chemicals Agency) in accordance with the REACH(Registration, Evaluation, Authorization and Restriction of Chemicals) policy and regulation. More and more scientific research has focused on the substitution of CH2Cl2, DMF and NMP in SPPS. In order to ensure that the newly developed methods can be used for mass production, the matter has not been looked at from an industrial production perspective
As the size and complexity of peptide drugs increase year by year, the application of ligation techniques such as expressed protein ligation or chemical enzyme ligation to merge smaller peptide fragments may become more important to meet the needs of production. Although the choice of this ligation method to synthesize peptides on a large scale has always been small, the green and cost have encouraged better development.
In conclusion, the development of green peptides is promising, especially for solvent substitution of SPPS. To this end, we have been investing significant resources in the development of a temporary green SPPS platform and are exploring a range of alternative platforms for peptide synthesis, which we will evaluate when necessary.
Post time: Jan-31-2024