During the peptide synthesis, some salt needs to be added. But there are many kinds of salt, and different types of salt make different peptides, and the effect is not the same. So today we mainly choose the appropriate type of peptide salt in peptide synthesis.
1. Trifluoroacetate (TFA) : This is a salt commonly used in peptide products, but it needs to be avoided in some experiments due to the biotoxicity of trifluoroacetate. For example, cell experiments.
2. Acetate (AC) : The biotoxicity of acetic acid is much less than trifluoroacetic acid, so most pharmaceutical and cosmetic peptides use acetate, but some products have unstable acetate, so the stability of the sequence also needs to be considered. Acetate was chosen for most cell experiments.
3. Hydrochloric acid (HCL) : This salt is rarely selected, and only some sequences use hydrochloric acid for special purposes.
4. Ammonium salt (NH4+) : This salt will seriously affect the solubility and stability of the product, must be selected in order.
5. Sodium salt (NA+) : it generally affects the stability and solubility of the product.
6. Pamoicacid: This salt is often used in peptide drugs to make sustained-release agents.
7. CitricAcid: This salt has relatively little physiological toxicity, but its preparation is very complex, so the production process needs to be developed sequentially and separately.
8. Salicylicacid: Salicylate can affect the stability of peptide products, so it is rarely used.
The above are several types of peptide salts, and we should also choose according to the characteristics of different salts in the actual use.
Post time: Jun-16-2023